Latest App Notes & Case Studies

This application note discusses the use of a Thermo Scientific Finnpipette Novus Electronic Pipette for the preparation of standard curves. With 10 different functions, the versatile Novus Electronic pipette can perform most every standard curve required. The pipette’s performance was compared to a manual Thermo Scientific Finnpipette model to determine if there were appreciable differences in pipetting speed and accuracy during curve preparation.

The BD™ Cytometric Bead Array (CBA) Cell Signaling Flex Set system lets you achieve high productivity in cell signaling studies by extracting more data from less sample faster than traditional Western blot techniques.

Accurate determination of live, dead, and total bacteria is important in many microbiology applications. Traditionally, viability in bacteria is synonymous with the ability to form colonies on solid growth medium and to proliferate in liquid nutrient broths. These traditional, culture-based tests are time-consuming and can work poorly with slow-growing or viable, but non-culturable organisms.

CellometerTM Auto T4 is routinely used for counting cells used in immunology research. This application note provides some details on methods of using the CellometerTM system, and shows images and counting data to illustrate a typical application. Viability determination is based on trypan blue dye exclusion. Cell concentration is determined automatically based on total cell count and dilution factor. Cell size exclusion is used to count mature DC cells cultured from PBMC.

Plasmacytoid dendritic cells are principal players in the innate immune response to viruses. Although rare (less than 0.2% of circulating peripheral blood cells), they secrete over 3 pg of the potent antiviral cytokine IFN-a per cell in response to HSV, mainly via TLR-mediated signaling upon recognition of viral DNA motifs.

Molecular translocation of transcription factors from the cytoplasm to the nucleus is a pivotal event in many processes critical to cellular activation, diff erentiation and host defense. Existing methods used to measure nuclear translocation have significant limitations.